Hotspot

trnS-psbZ

LSC · 35538-35876 · 339 bp

Back to Xanthium

Pi 0.0236

MarkerSeek score 66.90

Primer available yes

Label trnS-psbZ

Feature Metadata

label_name: trnS-psbZ
feature_type: igs
region: LSC
length_bp: 339
variable_sites: 8
pi: 0.0236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-psbZ_p1	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	459-466 bp	1.0	79.6
trnS-psbZ_p2	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	55.0	55.0	58.0	58.3	461-468 bp	1.0	79.2
trnS-psbZ_p3	TCAACCACTCAGCCATCTCT	CCAACCATCAGGAGAAGCAA	50.0	50.0	58.4	57.8	460-467 bp	1.0	76.9
trnS-psbZ_p4	TCAACCACTCAGCCATCTCT	GACCAACCATCAGGAGAAGC	50.0	55.0	58.4	58.3	462-469 bp	1.0	76.5
trnS-psbZ_p5	TTCAACCACTCAGCCATCTC	CCAACCATCAGGAGAAGCAA	50.0	50.0	57.5	57.8	461-468 bp	1.0	75.9