Hotspot

atpI-atpH

LSC · 25491-26660 · 1170 bp

Back to Xanthium

Pi 0.0129

MarkerSeek score 67.10

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1170
variable_sites: 15
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	CCCGTCATGTTCCTTGGATT	ATAACGGAAGCGGCAGAAAT	50.0	45.0	57.9	58.0	1294-1304 bp	1.0	80.3
atpI-atpH_p2	CCCGTCATGTTCCTTGGATT	TACCTTGACCAACTCCAGGT	50.0	50.0	57.9	57.9	1350-1360 bp	1.0	79.6
atpI-atpH_p3	CCCGTCATGTTCCTTGGATT	AGCCAATCCAGCAGCAATAA	50.0	45.0	57.9	57.9	1310-1320 bp	1.0	79.4
atpI-atpH_p4	CCCGTCATGTTCCTTGGATT	AATAACGGAAGCGGCAGAAA	50.0	45.0	57.9	58.2	1295-1305 bp	1.0	79.1
atpI-atpH_p5	CCCGTCATGTTCCTTGGATT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.9	58.2	1354-1364 bp	1.0	79.0