Hotspot

trnE-rpoB

LSC · 12365-13276 · 912 bp

Back to Xanthium

Pi 0.0156

MarkerSeek score 59.60

Primer available yes

Label trnE-rpoB

Feature Metadata

label_name: trnE-rpoB
feature_type: igs
region: LSC
length_bp: 912
variable_sites: 14
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-rpoB_p1	GGGACTTGAACCCTCGTTTT	TGCCAAACCCTGATCAATGA	50.0	45.0	58.0	57.4	1035-1046 bp	1.0	77.2
trnE-rpoB_p2	TAACCACTGGACCATAGGGG	TGCCAAACCCTGATCAATGA	55.0	45.0	58.1	57.4	994-1005 bp	1.0	76.5
trnE-rpoB_p3	CCCTCGTTTTCTCCGTGAAA	TGCCAAACCCTGATCAATGA	50.0	45.0	58.1	57.4	1025-1036 bp	1.0	76.4
trnE-rpoB_p4	TTGAACCCTCGTTTTCTCCG	TGCCAAACCCTGATCAATGA	50.0	45.0	58.1	57.4	1030-1041 bp	1.0	76.3
trnE-rpoB_p5	TCTTGGTATACCCCCAGAGG	TGCCAAACCCTGATCAATGA	55.0	45.0	57.5	57.4	1053-1064 bp	1.0	73.4