Hotspot

psbM-trnD

LSC · 11185-11842 · 658 bp

Back to Xanthium

Pi 0.0213

MarkerSeek score 63.10

Primer available yes

Label psbM-trnD

Feature Metadata

label_name: psbM-trnD
feature_type: igs
region: LSC
length_bp: 658
variable_sites: 14
pi: 0.0213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD_p1	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.6	58.3	775-777 bp	1.0	51.8
psbM-trnD_p2	AAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	40.0	50.0	54.5	58.3	769-771 bp	1.0	51.4
psbM-trnD_p3	ACTAAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.4	58.3	772-774 bp	1.0	50.4
psbM-trnD_p4	AGGAACTAAAATGAACAGTGCAG	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.3	58.3	776-778 bp	1.0	49.8
psbM-trnD_p5	AGGAACTAAAATGAACAGTGCA	TTCAATAGGCAAGAGCACCG	36.4	50.0	56.2	58.3	776-778 bp	1.0	48.9