Hotspot

trnC-petN

LSC · 9676-10493 · 818 bp

Back to Xanthium

Pi 0.0186

MarkerSeek score 70.80

Primer available yes

Label trnC-petN

Feature Metadata

label_name: trnC-petN
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 15
pi: 0.0186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	863-874 bp	1.0	80.6
trnC-petN_p2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	881-892 bp	1.0	78.4
trnC-petN_p3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	862-873 bp	1.0	74.7
trnC-petN_p4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.1	58.9	871-882 bp	1.0	74.6
trnC-petN_p5	ATTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.1	58.2	920-931 bp	1.0	73.8