Hotspot

psbI-trnS

LSC · 8614-8769 · 156 bp

Back to Xanthium

Pi 0.0467

MarkerSeek score 74.80

Primer available yes

Label psbI-trnS

Feature Metadata

label_name: psbI-trnS
feature_type: igs
region: LSC
length_bp: 156
variable_sites: 7
pi: 0.0467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS_p1	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.1	267-272 bp	1.0	75.5
psbI-trnS_p2	CGTAATCCTGGACGTGAAGA	CGAGTTATTCGTACCGAGGG	50.0	55.0	57.1	57.6	215-220 bp	1.0	74.1
psbI-trnS_p3	CGTAATCCTGGACGTGAAGAA	ATTGGGAGAGATGGCTGAGT	47.6	50.0	57.8	58.1	267-272 bp	1.0	73.2
psbI-trnS_p4	CCTATCCAATGATCCAGGACG	ATTGGGAGAGATGGCTGAGT	52.4	50.0	57.7	58.1	286-291 bp	1.0	72.1
psbI-trnS_p5	CGTAATCCTGGACGTGAAGA	GATGGCTGAGTGGACGAAAG	50.0	55.0	57.1	58.6	258-263 bp	1.0	71.4