Hotspot

rps15-ycf1

SSC · 131715-133269 · 1555 bp

Back to Wurfbainia

Pi 0.00897

MarkerSeek score 54.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 1555
variable_sites: 31
pi: 0.00897
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACGGAACCTCTGTTTTCT	TTTTGATTGGATGGGAATGA	42.9	35.0	57.4	52.7	1593-1648 bp	1.0	44.6
rps15-ycf1_p2	TCAACGGAACCTCTGTTTTCT	TTTTTGATTGGATGGGAATGA	42.9	33.3	57.4	53.6	1594-1649 bp	1.0	44.6
rps15-ycf1_p3	ACGGAACCTCTGTTTTCTTCT	TTTTGATTGGATGGGAATGA	42.9	35.0	57.2	52.7	1590-1645 bp	1.0	44.6
rps15-ycf1_p4	ACGGAACCTCTGTTTTCTTCT	TTTTTGATTGGATGGGAATGA	42.9	33.3	57.2	53.6	1591-1646 bp	1.0	44.6
rps15-ycf1_p5	TCAACGGAACCTCTGTTTTC	TTTTGATTGGATGGGAATGA	45.0	35.0	55.9	52.7	1593-1648 bp	1.0	44.6