Hotspot

ndhE-ndhG

SSC · 126565-126773 · 209 bp

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Pi 0.00882

MarkerSeek score 54.00

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 209
variable_sites: 4
pi: 0.00882
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	AAGTACATGCTCGAACATCA	AGGTGTAATTACTATGGCTCGC	40.0	45.5	54.8	58.0	252-258 bp	1.0	43.2
ndhE-ndhG_p2	GTTTCGACTTGTGATCAATCCA	AGGTGTAATTACTATGGCTCGC	40.9	45.5	57.0	58.0	309-315 bp	1.0	42.6
ndhE-ndhG_p3	TGTTTCGACTTGTGATCAATCC	AGGTGTAATTACTATGGCTCGC	40.9	45.5	57.0	58.0	310-316 bp	1.0	42.6
ndhE-ndhG_p4	TTCGACTTGTGATCAATCCA	AGGTGTAATTACTATGGCTCGC	40.0	45.5	54.7	58.0	307-313 bp	1.0	42.6
ndhE-ndhG_p5	AGTGCTCTAATCATGTTTCGACT	AGGTGTAATTACTATGGCTCGC	39.1	45.5	57.9	58.0	323-329 bp	1.0	42.5