Hotspot

ndhJ-ndhK

LSC · 52637-52740 · 104 bp

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Pi 0.00962
MarkerSeek score 52.60
Primer available yes
Label ndhJ-ndhK

Feature Metadata

label_name
ndhJ-ndhK
feature_type
igs
region
LSC
length_bp
104
variable_sites
1
pi
0.00962
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhJ-ndhK_p1 GGAATCCCAATCCTCGGTTT CCCCCGAATTAGTGAATCAGG 50.0 52.4 57.6 58.1 248-251 bp 1.0 68.3
ndhJ-ndhK_p2 GCAATCGAAGCCCAAAGATC CCCCCGAATTAGTGAATCAGG 50.0 52.4 57.5 58.1 203-206 bp 1.0 68.1
ndhJ-ndhK_p3 TGGAATCCCAATCCTCGGTT CCCCCGAATTAGTGAATCAGG 50.0 52.4 58.7 58.1 249-252 bp 1.0 66.1
ndhJ-ndhK_p4 GGCAATCGAAGCCCAAAGAT CCCCCGAATTAGTGAATCAGG 50.0 52.4 58.9 58.1 204-207 bp 1.0 64.8
ndhJ-ndhK_p5 TGGAATCCCAATCCTCGGT CCCCCGAATTAGTGAATCAGG 52.6 52.4 58.0 58.1 249-252 bp 1.0 63.6