Hotspot

psbC-trnS-TGA

LSC · 38264-38408 · 145 bp

Back to Wurfbainia

Pi 0.0372

MarkerSeek score 55.80

Primer available yes

Label psbC-trnS-TGA

Feature Metadata

label_name: psbC-trnS-TGA
feature_type: igs
region: LSC
length_bp: 145
variable_sites: 8
pi: 0.0372
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-TGA_p1	CTTTCCATGACCCCTCTTCG	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.0	58.0	258-261 bp	1.0	83.0
psbC-trnS-TGA_p2	CATTTGTGGCATGCAGGAAG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.0	58.0	336-339 bp	1.0	82.4
psbC-trnS-TGA_p3	CGACGTTCGGCGGAATATAT	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.0	58.0	489-492 bp	1.0	81.4
psbC-trnS-TGA_p4	CGTTTTTGGGATCTTCGTGC	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.0	58.0	588-591 bp	1.0	81.2
psbC-trnS-TGA_p5	CATTTGTGGCATGCAGGAAG	CGGGTCGGGTAAATGGAAAT	50.0	50.0	58.0	58.0	934-949 bp	1.0	81.1