Hotspot

psbM-trnD-GTC

LSC · 31706-32791 · 1086 bp

Back to Wurfbainia

Pi 0.00517

MarkerSeek score 43.40

Primer available yes

Label psbM-trnD-GTC

Feature Metadata

label_name: psbM-trnD-GTC
feature_type: igs
region: LSC
length_bp: 1086
variable_sites: 14
pi: 0.00517
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GTC_p1	AGAATGAACAGTGCAGTAGC	CCTCGCTGGGATTGTAGTTC	45.0	55.0	55.8	58.1	1212-1214 bp	1.0	62.8
psbM-trnD-GTC_p2	AGAATGAACAGTGCAGTAGC	TACACCTCGCTGGGATTGTA	45.0	50.0	55.8	58.1	1216-1218 bp	1.0	62.1
psbM-trnD-GTC_p3	AGAATGAACAGTGCAGTAGC	CATACACCTCGCTGGGATTG	45.0	55.0	55.8	58.4	1218-1220 bp	1.0	60.0
psbM-trnD-GTC_p4	AGGAACTAGAATGAACAGTGCA	CCTCGCTGGGATTGTAGTTC	40.9	55.0	57.4	58.1	1219-1221 bp	1.0	59.7
psbM-trnD-GTC_p5	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1195-1197 bp	1.0	59.5