Hotspot

rps12-ICG91_pgr003

IRa · 139846-142768 · 2923 bp

Back to Woodwardia

Pi 0.089

MarkerSeek score 82.20

Primer available yes

Label rps12-ICG91_pgr003

Feature Metadata

label_name: rps12-ICG91_pgr003
feature_type: igs
region: IRa
length_bp: 2923
variable_sites: 385
pi: 0.089
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-ICG91_pgr003_p1	GAACTCTCGCGACTTTACGT	AGCCAGGATCAAACTCTCCA	50.0	50.0	58.0	58.3	3000 bp	0.333333	50.3
rps12-ICG91_pgr003_p2	CTCTCGCGACTTTACGTAGG	GAGCCAGGATCAAACTCTCC	55.0	55.0	57.9	57.7	2998 bp	0.333333	50.0
rps12-ICG91_pgr003_p3	CTCTCGCGACTTTACGTAGG	AGCCAGGATCAAACTCTCCA	55.0	50.0	57.9	58.3	2997 bp	0.333333	49.9
rps12-ICG91_pgr003_p4	CGACTTTACGTAGGGCAGAG	GAGCCAGGATCAAACTCTCC	55.0	55.0	57.8	57.7	2992 bp	0.333333	49.2
rps12-ICG91_pgr003_p5	CGACTTTACGTAGGGCAGAG	AGCCAGGATCAAACTCTCCA	55.0	50.0	57.8	58.3	2991 bp	0.333333	49.1