Hotspot

ccsA-ndhD

SSC · 112483-112895 · 413 bp

Back to Woodwardia

Pi 0.0675

MarkerSeek score 79.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 413
variable_sites: 40
pi: 0.0675
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGCAGTCTCATCGGTCAATG	ATTTTGGTCCGAGGGAGGTA	50.0	50.0	58.0	58.0	1088-1107 bp	1.0	87.3
ccsA-ndhD_p2	TGCAGTCTCATCGGTCAATG	TTTGGTCCGAGGGAGGTATT	50.0	50.0	58.0	58.0	1086-1105 bp	1.0	87.3
ccsA-ndhD_p3	TGCAGTCTCATCGGTCAATG	TTTTGGTCCGAGGGAGGTAT	50.0	50.0	58.0	58.0	1087-1106 bp	1.0	87.3
ccsA-ndhD_p4	TGCAGTCTCATCGGTCAATG	AGCGTTATTCTCCCTTGCAG	50.0	50.0	58.0	58.0	1426-1445 bp	1.0	86.4
ccsA-ndhD_p5	GTTTGCCTCTTGGGAGTCAA	AGCGTTATTCTCCCTTGCAG	50.0	50.0	58.0	58.0	942-954 bp	0.666667	72.1