Hotspot

rpl32-trnP-GGG

SSC · 110293-110829 · 537 bp

Back to Woodwardia

Pi 0.102
MarkerSeek score 80.00
Primer available yes
Label rpl32-trnP-GGG

Feature Metadata

label_name
rpl32-trnP-GGG
feature_type
igs
region
SSC
length_bp
537
variable_sites
79
pi
0.102
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnP-GGG_p1 ATGCAGTTGTCAAAGGCAGA GAGGTCACAGGTTCGAATCC 45.0 55.0 58.0 58.0 1086-1094 bp 1.0 89.6
rpl32-trnP-GGG_p2 ATGCAGTTGTCAAAGGCAGA GGAGGTCACAGGTTCGAATC 45.0 55.0 58.0 58.0 1087-1095 bp 1.0 89.6
rpl32-trnP-GGG_p3 TGCGTTAAGGGCTTTTTCCT CTGGCACTGCTTCCTAAGAG 45.0 55.0 58.0 58.0 1008-1018 bp 1.0 89.6
rpl32-trnP-GGG_p4 TGCGTTAAGGGCTTTTTCCT GAGGTCACAGGTTCGAATCC 45.0 55.0 58.0 58.0 656-677 bp 1.0 89.1
rpl32-trnP-GGG_p5 TGCGTTAAGGGCTTTTTCCT GGAGGTCACAGGTTCGAATC 45.0 55.0 58.0 58.0 657-678 bp 1.0 89.1