Hotspot

rpl21-rpl32

SSC · 109945-110124 · 180 bp

Back to Woodwardia

Pi 0.0685

MarkerSeek score 80.00

Primer available yes

Label rpl21-rpl32

Feature Metadata

label_name: rpl21-rpl32
feature_type: igs
region: SSC
length_bp: 180
variable_sites: 18
pi: 0.0685
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl21-rpl32_p1	ATGCAGTTGTCAAAGGCAGA	GAGGTCACAGGTTCGAATCC	45.0	55.0	58.0	58.0	1086-1094 bp	1.0	89.6
rpl21-rpl32_p2	ATGCAGTTGTCAAAGGCAGA	GGAGGTCACAGGTTCGAATC	45.0	55.0	58.0	58.0	1087-1095 bp	1.0	89.6
rpl21-rpl32_p3	ATGCAGTTGTCAAAGGCAGA	AGGAAAAAGCCCTTAACGCA	45.0	45.0	58.0	58.0	437-450 bp	1.0	87.5
rpl21-rpl32_p4	CAATAATTGACATCGGCGGC	AGGAAAAAGCCCTTAACGCA	50.0	45.0	58.0	58.0	611-624 bp	1.0	86.7
rpl21-rpl32_p5	GCAATAATTGACATCGGCGG	AGGAAAAAGCCCTTAACGCA	50.0	45.0	58.0	58.0	612-625 bp	1.0	86.7