Hotspot

trnR-UCU-rps12

IRb · 92937-95859 · 2923 bp

Back to Woodwardia

Pi 0.0893

MarkerSeek score 82.20

Primer available yes

Label trnR-UCU-rps12

Feature Metadata

label_name: trnR-UCU-rps12
feature_type: igs
region: IRb
length_bp: 2923
variable_sites: 386
pi: 0.0893
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-rps12_p1	AGCCAGGATCAAACTCTCCA	GAACTCTCGCGACTTTACGT	50.0	50.0	58.3	58.0	3000 bp	0.333333	50.3
trnR-UCU-rps12_p2	GAGCCAGGATCAAACTCTCC	CTCTCGCGACTTTACGTAGG	55.0	55.0	57.7	57.9	2998 bp	0.333333	50.0
trnR-UCU-rps12_p3	AGCCAGGATCAAACTCTCCA	CTCTCGCGACTTTACGTAGG	50.0	55.0	58.3	57.9	2997 bp	0.333333	49.9
trnR-UCU-rps12_p4	GAGCCAGGATCAAACTCTCC	CGACTTTACGTAGGGCAGAG	55.0	55.0	57.7	57.8	2992 bp	0.333333	49.2
trnR-UCU-rps12_p5	AGCCAGGATCAAACTCTCCA	CGACTTTACGTAGGGCAGAG	50.0	55.0	58.3	57.8	2991 bp	0.333333	49.1