Hotspot

petA-psbJ

LSC · 59657-60488 · 832 bp

Back to Woodwardia

Pi 0.0603

MarkerSeek score 80.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 832
variable_sites: 72
pi: 0.0603
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGTTGGTGGATTTGGTCAGG	TCGTGGTTGGTAGGTACTGT	50.0	50.0	58.0	58.0	1094-1122 bp	1.0	87.1
petA-psbJ_p2	GTATCCAGGGTCTCGTAGCT	TCGTGGTTGGTAGGTACTGT	55.0	50.0	58.0	58.0	1041-1069 bp	1.0	87.1
petA-psbJ_p3	GGGCAAATCTACCCAGATGG	TCGTGGTTGGTAGGTACTGT	55.0	50.0	58.0	58.0	1324-1352 bp	1.0	86.6
petA-psbJ_p4	TAATCGCATTCCAGCCGAAA	TCGTGGTTGGTAGGTACTGT	45.0	50.0	58.0	58.0	1535-1563 bp	1.0	86.0
petA-psbJ_p5	ATCGTTTCAGAGTTACCGCC	TCGTGGTTGGTAGGTACTGT	50.0	50.0	58.0	58.0	1493 bp	0.333333	53.3