Hotspot

petN-trnC-GCA

LSC · 29268-29750 · 483 bp

Back to Woodwardia

Pi 0.0605

MarkerSeek score 80.60

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 483
variable_sites: 42
pi: 0.0605
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	GCCCAAGCTATAGTAACTAGATCC	AAGACGGAGGACTGCAAATC	45.8	50.0	58.0	57.9	557-565 bp	1.0	55.3
petN-trnC-GCA_p2	GCCCAAGCTATAGTAACTAGATCC	CCAGTTCGAATCTGGGTGTC	45.8	55.0	58.0	58.3	528-536 bp	1.0	54.4
petN-trnC-GCA_p3	CCCAAGCTATAGTAACTAGATCCG	AAGACGGAGGACTGCAAATC	45.8	50.0	57.8	57.9	556-564 bp	1.0	54.0
petN-trnC-GCA_p4	GCCCAAGCTATAGTAACTAGATCC	TCAAGTGGTAAGACGGAGGA	45.8	50.0	58.0	57.7	566-574 bp	1.0	53.6
petN-trnC-GCA_p5	GCCCAAGCTATAGTAACTAGATCC	AAGTGGTAAGACGGAGGACT	45.8	50.0	58.0	57.7	564-572 bp	1.0	53.6