Hotspot

rpoB-trnD-GUC

LSC · 26519-27309 · 791 bp

Back to Woodwardia

Pi 0.0489

MarkerSeek score 84.90

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: LSC
length_bp: 791
variable_sites: 57
pi: 0.0489
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	CAAGTTCTGGTAGTACGGGC	GCGGTGCTCTAACCAATTGA	55.0	50.0	58.0	58.6	829-911 bp	1.0	83.4
rpoB-trnD-GUC_p2	GCAAGTTCTGGTAGTACGGG	GCGGTGCTCTAACCAATTGA	55.0	50.0	58.0	58.6	830-912 bp	1.0	83.4
rpoB-trnD-GUC_p3	AGTTCTGGTAGTACGGGCAT	GCGGTGCTCTAACCAATTGA	50.0	50.0	58.1	58.6	827-909 bp	1.0	82.4
rpoB-trnD-GUC_p4	AAGTTCTGGTAGTACGGGCA	GCGGTGCTCTAACCAATTGA	50.0	50.0	58.4	58.6	828-910 bp	1.0	80.6
rpoB-trnD-GUC_p5	CAAGTTCTGGTAGTACGGGC	GGATTGACGGGTCTCGAAC	55.0	57.9	58.0	58.0	870-952 bp	1.0	79.1