Hotspot

atpH-atpI

LSC · 13519-14271 · 753 bp

Back to Woodwardia

Pi 0.0514

MarkerSeek score 81.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 753
variable_sites: 56
pi: 0.0514
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AGATAGATGCGGCAGGTACT	GTTGGTAGTAGCTGTTCCCG	50.0	55.0	58.0	58.0	1286-1294 bp	1.0	86.8
atpH-atpI_p2	AGATAGATGCGGCAGGTACT	GTACCCATGACGCCTTTAGG	50.0	55.0	58.0	58.1	1240-1248 bp	1.0	86.5
atpH-atpI_p3	AGATAGATGCGGCAGGTACT	TCTACCAAACCCCCATCACT	50.0	50.0	58.0	57.9	1342-1350 bp	1.0	86.2
atpH-atpI_p4	AGATAGATGCGGCAGGTACT	GGCGAATCGTTCAGTTACCT	50.0	50.0	58.0	58.0	1530-1538 bp	0.666667	71.6
atpH-atpI_p5	AGATAGATGCGGCAGGTACT	ATCAACACTACTGTCGCGTC	50.0	50.0	58.0	58.0	1488 bp	0.333333	53.1