Hotspot

ycf1-chlN

Genome · 124880-125230 · 351 bp

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Pi 0.0771

MarkerSeek score 71.20

Primer available yes

Label ycf1-chlN

Feature Metadata

label_name: ycf1-chlN
feature_type: igs
region: Genome
length_bp: 351
variable_sites: 27
pi: 0.0771
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-chlN_p1	AAGAGCAGGTGGGTTAGTCT	GTCCATTGCGACGTAGAAGT	50.0	50.0	58.0	58.0	880-890 bp	1.0	84.7
ycf1-chlN_p2	CCGGAGCTCTACCCAGATAA	GTCCATTGCGACGTAGAAGT	55.0	50.0	58.0	58.0	1092-1102 bp	1.0	84.6
ycf1-chlN_p3	CCGGAGCTCTACCCAGATAA	CCATTGCGACGTAGAAGTGA	55.0	50.0	58.0	58.0	1090-1100 bp	1.0	84.6
ycf1-chlN_p4	CCGGAGCTCTACCCAGATAA	TCCATTGCGACGTAGAAGTG	55.0	50.0	58.0	58.0	1091-1101 bp	1.0	84.6
ycf1-chlN_p5	CCGGAGCTCTACCCAGATAA	AGTTACTCGTCCATTGCGAC	55.0	50.0	58.0	58.0	1100 bp	0.5	59.9