Hotspot

trnP-UGG-psaJ

Genome · 62878-63234 · 357 bp

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Pi 0.0702

MarkerSeek score 71.70

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: Genome
length_bp: 357
variable_sites: 25
pi: 0.0702
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACGAAACATACATGAGGGGC	AACGTCTACGGAGAGATCGT	50.0	50.0	58.0	58.0	1481 bp	0.5	59.5
trnP-UGG-psaJ_p2	ACGAAACATACATGAGGGGC	ACGTCTACGGAGAGATCGTT	50.0	50.0	58.0	58.0	1480 bp	0.5	59.5
trnP-UGG-psaJ_p3	ATACCCACAATATCGACGCG	AACGTCTACGGAGAGATCGT	50.0	50.0	57.9	58.0	1671 bp	0.5	59.3
trnP-UGG-psaJ_p4	ATACCCACAATATCGACGCG	ACGTCTACGGAGAGATCGTT	50.0	50.0	57.9	58.0	1670 bp	0.5	59.3
trnP-UGG-psaJ_p5	ACGAAACATACATGAGGGGC	CATCAGAGCGAATAGACGGG	50.0	55.0	58.0	57.9	1502 bp	0.5	59.2