Hotspot

psbE-petL

Genome · 61169-61902 · 734 bp

Back to Woodsia

Pi 0.045

MarkerSeek score 77.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 734
variable_sites: 33
pi: 0.045
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	GCGTTCTCCTGTATTCCCAG	GGGTCAAAGTTGAAACAGACG	55.0	47.6	58.1	57.7	813 bp	1.0	72.2
psbE-petL_p2	GCGTTCTCCTGTATTCCCAG	TGAAACAGACGTCAAAAAGGC	55.0	42.9	58.1	57.6	803 bp	1.0	71.6
psbE-petL_p3	GCGTTCTCCTGTATTCCCAG	GTCAAAAAGGCGAAGTAGCT	55.0	45.0	58.1	56.4	793 bp	1.0	69.9
psbE-petL_p4	GTAATGCTGTGGATGACCCA	GGGTCAAAGTTGAAACAGACG	50.0	47.6	57.6	57.7	866 bp	1.0	69.1
psbE-petL_p5	GTAATGCTGTGGATGACCCA	TGAAACAGACGTCAAAAAGGC	50.0	42.9	57.6	57.6	856 bp	1.0	68.5