Hotspot

trnT-GGU-trnfM-CAU

Genome · 35284-35772 · 489 bp

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Pi 0.0613

MarkerSeek score 76.40

Primer available yes

Label trnT-GGU-trnfM-CAU

Feature Metadata

label_name: trnT-GGU-trnfM-CAU
feature_type: igs
region: Genome
length_bp: 489
variable_sites: 30
pi: 0.0613
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnfM-CAU_p1	CCATGGCGTTACTCTACCAC	GTTCAAATCCTGTCTCCGCA	55.0	50.0	58.1	58.2	542-549 bp	1.0	82.4
trnT-GGU-trnfM-CAU_p2	CCATGGCGTTACTCTACCAC	TAACTAAGCGGGGTAGAGCA	55.0	50.0	58.1	57.9	601-608 bp	1.0	82.3
trnT-GGU-trnfM-CAU_p3	TGGCGTTACTCTACCACTGA	GTTCAAATCCTGTCTCCGCA	50.0	50.0	58.1	58.2	539-546 bp	1.0	82.2
trnT-GGU-trnfM-CAU_p4	TGGCGTTACTCTACCACTGA	TAACTAAGCGGGGTAGAGCA	50.0	50.0	58.1	57.9	598-605 bp	1.0	82.1
trnT-GGU-trnfM-CAU_p5	CCATGGCGTTACTCTACCAC	TAGCTCGCAAGGCTCATAAC	55.0	50.0	58.1	57.8	575-582 bp	1.0	81.8