Hotspot

trnC-GCA-trnG-GCC

Genome · 29714-30162 · 449 bp

Back to Woodsia

Pi 0.0784

MarkerSeek score 77.60

Primer available yes

Label trnC-GCA-trnG-GCC

Feature Metadata

label_name: trnC-GCA-trnG-GCC
feature_type: igs
region: Genome
length_bp: 449
variable_sites: 32
pi: 0.0784
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnG-GCC_p1	TCTTACCACTTGACTATATCGCC	GTTCGATTCCCGCTATCCG	43.5	57.9	57.6	58.2	475-493 bp	1.0	52.2
trnC-GCA-trnG-GCC_p2	TCTTACCACTTGACTATATCGCC	GGGTTCGATTCCCGCTATC	43.5	57.9	57.6	57.8	477-495 bp	1.0	52.0
trnC-GCA-trnG-GCC_p3	TCTTACCACTTGACTATATCGCC	GGTTCGATTCCCGCTATCC	43.5	57.9	57.6	57.8	476-494 bp	1.0	52.0
trnC-GCA-trnG-GCC_p4	CTTACCACTTGACTATATCGCC	GTTCGATTCCCGCTATCCG	45.5	57.9	56.2	58.2	474-492 bp	1.0	49.3
trnC-GCA-trnG-GCC_p5	CTTACCACTTGACTATATCGCC	GGTTCGATTCCCGCTATCC	45.5	57.9	56.2	57.8	475-493 bp	1.0	49.3