Hotspot

trnE-UUC-psbM

Genome · 27689-28124 · 436 bp

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Pi 0.113

MarkerSeek score 81.80

Primer available yes

Label trnE-UUC-psbM

Feature Metadata

label_name: trnE-UUC-psbM
feature_type: igs
region: Genome
length_bp: 436
variable_sites: 47
pi: 0.113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-psbM_p1	TCGAGAAGGAATACCCCCAG	ACGCAAGAATATTTACTTCCGT	55.0	36.4	58.2	56.2	537-542 bp	1.0	57.9
trnE-UUC-psbM_p2	TTCGAGAAGGAATACCCCCA	ACGCAAGAATATTTACTTCCGT	50.0	36.4	57.7	56.2	538-543 bp	1.0	57.5
trnE-UUC-psbM_p3	TCGAGAAGGAATACCCCCAG	AACGCAAGAATATTTACTTCCGT	55.0	34.8	58.2	56.9	538-543 bp	1.0	55.4
trnE-UUC-psbM_p4	TTCGAGAAGGAATACCCCCA	AACGCAAGAATATTTACTTCCGT	50.0	34.8	57.7	56.9	539-544 bp	1.0	55.0
trnE-UUC-psbM_p5	TCGAGAAGGAATACCCCCAG	AAACGCAAGAATATTTACTTCCGT	55.0	33.3	58.2	57.5	539-544 bp	1.0	52.4