Hotspot

atpF-atpH

Genome · 12951-13290 · 340 bp

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Pi 0.0735

MarkerSeek score 77.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: Genome
length_bp: 340
variable_sites: 25
pi: 0.0735
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CGGCAAGAGTCCAATCACTT	GGATCAGTTGTAGCTCCAGC	50.0	55.0	58.2	58.1	440-446 bp	1.0	82.6
atpF-atpH_p2	GTCCAATCACTTGAGGGAGC	GGATCAGTTGTAGCTCCAGC	55.0	55.0	58.3	58.1	432-438 bp	1.0	82.1
atpF-atpH_p3	CGGCGATCTCACTTACACTT	GGATCAGTTGTAGCTCCAGC	50.0	55.0	57.7	58.1	413-419 bp	1.0	82.1
atpF-atpH_p4	CGGCAAGAGTCCAATCACTT	TCCGTCATTTGCCAATCCTT	50.0	45.0	58.2	57.8	420-426 bp	1.0	81.5
atpF-atpH_p5	CGGCAAGAGTCCAATCACTT	CGGATCAGTTGTAGCTCCAG	50.0	55.0	58.2	57.8	441-447 bp	1.0	81.3