Hotspot

ndhH

SSC · 124140-125327 · 1188 bp

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Pi 0.309

MarkerSeek score 77.30

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1188
variable_sites: 543
pi: 0.309
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	ACTTCCCATTTGGAGTTGCA	TCAGGTCCAATACGCTGTTG	45.0	50.0	57.6	57.9	1705-1708 bp	0.5	62.1
ndhH_p2	ACTTCCCATTTGGAGTTGCA	CAGGTCCAATACGCTGTTGT	45.0	50.0	57.6	58.2	1704-1707 bp	0.5	61.3
ndhH_p3	ACTTCCCATTTGGAGTTGCA	CCCCCTAGATGGAAGGACAT	45.0	55.0	57.6	57.9	1793 bp	0.25	46.0
ndhH_p4	ACTTCCCATTTGGAGTTGCA	ATACGACTGCAATAGCTGGC	45.0	50.0	57.6	58.1	1836 bp	0.25	45.9
ndhH_p5	ACTTCCCATTTGGAGTTGCA	CTGTTGTATCGCGGCAGATA	45.0	50.0	57.6	57.9	1694 bp	0.25	45.9