Hotspot

rpl32-ndhF

SSC · 134274-135314 · 1041 bp

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Pi 0.325

MarkerSeek score 74.70

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1041
variable_sites: 434
pi: 0.325
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.66
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ACTGTCCTGTGGAGATCGAT	CCCACATACGACGACTGTTT	50.0	50.0	57.8	57.9	1281-1289 bp	0.5	70.2
rpl32-ndhF_p2	ACTGTCCTGTGGAGATCGAT	AAGGGGAAATTGTGCGGATT	50.0	45.0	57.8	58.1	1892-1900 bp	0.5	69.2
rpl32-ndhF_p3	ACTGTCCTGTGGAGATCGAT	AGGGGAAATTGTGCGGATTT	50.0	45.0	57.8	58.1	1891-1899 bp	0.5	69.2
rpl32-ndhF_p4	ACTGTCCTGTGGAGATCGAT	TTGGTCGGGTGAACCAAAAA	50.0	45.0	57.8	58.2	1668-1676 bp	0.5	68.8
rpl32-ndhF_p5	ACTGTCCTGTGGAGATCGAT	AGGAAATGCCCACATACGAC	50.0	50.0	57.8	58.0	1297 bp	0.25	48.5