Hotspot

ndhE-psaC

SSC · 129452-130274 · 823 bp

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Pi 0.39

MarkerSeek score 77.50

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: SSC
length_bp: 823
variable_sites: 444
pi: 0.39
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	GTCGAAGCATGGTTAGAGCA	GAAAAATGAAACAGCCCGCA	50.0	45.0	58.0	57.9	1507-1510 bp	0.5	69.8
ndhE-psaC_p2	TCGAAGCATGGTTAGAGCAC	GAAAAATGAAACAGCCCGCA	50.0	45.0	58.0	57.9	1506-1509 bp	0.5	69.8
ndhE-psaC_p3	GTCGAAGCATGGTTAGAGCA	TATTTCCTCTGTGCGGGAGA	50.0	50.0	58.0	58.1	1528 bp	0.25	48.8
ndhE-psaC_p4	TCGAAGCATGGTTAGAGCAC	TATTTCCTCTGTGCGGGAGA	50.0	50.0	58.0	58.1	1527 bp	0.25	48.8
ndhE-psaC_p5	CGAAGCATGGTTAGAGCACT	TATTTCCTCTGTGCGGGAGA	50.0	50.0	58.0	58.1	1526 bp	0.25	48.8