Hotspot

rbcL-accD

LSC · 62693-63611 · 919 bp

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Pi 0.0862

MarkerSeek score 84.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 919
variable_sites: 135
pi: 0.0862
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TGCCTGGTGTTATCCCTGTA	TAACTCCCGCTCTCATTCCA	50.0	50.0	58.0	58.1	1286-1292 bp	0.5	60.7
rbcL-accD_p2	TGCACTGACCGAGATCTTTG	TAACTCCCGCTCTCATTCCA	50.0	50.0	57.9	58.1	1234-1240 bp	0.5	60.4
rbcL-accD_p3	GCACTACGTATGTCTGGTGG	TAACTCCCGCTCTCATTCCA	55.0	50.0	58.1	58.1	1449-1455 bp	0.5	60.2
rbcL-accD_p4	GGTGAGCGTGAGATGACTTT	TAACTCCCGCTCTCATTCCA	50.0	50.0	57.9	58.1	1389-1395 bp	0.5	60.2
rbcL-accD_p5	TTATCCGTGAAGCTGCGAAA	TAACTCCCGCTCTCATTCCA	45.0	50.0	57.9	58.1	1067 bp	0.25	48.2