Hotspot

trnT-GGU-psbD

LSC · 35697-36895 · 1199 bp

Back to Wolffia

Pi 0.0705

MarkerSeek score 90.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1199
variable_sites: 141
pi: 0.0705
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 90.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1288-1341 bp	1.0	91.5
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1291-1344 bp	1.0	91.3
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CACAAAAACGAAACGGTCCC	55.0	50.0	58.1	58.2	1297-1350 bp	1.0	90.1
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	CCACAAAAACGAAACGGTCC	55.0	50.0	58.1	58.2	1298-1351 bp	1.0	90.1
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CCCACAAAAACGAAACGGTC	55.0	50.0	58.1	58.2	1299-1352 bp	1.0	90.1