Hotspot

trnC-GCA-petN

LSC · 30165-31169 · 1005 bp

Back to Wolffia

Pi 0.105

MarkerSeek score 85.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1005
variable_sites: 170
pi: 0.105
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	776-1116 bp	1.0	87.0
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTA	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.5	58.2	801-1141 bp	1.0	86.6
trnC-GCA-petN_p3	GACATGGCCAAGTGGTAAGG	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.5	58.2	822-1162 bp	1.0	86.5
trnC-GCA-petN_p4	CGACATGGCCAAGTGGTAAG	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.9	58.2	823-1163 bp	1.0	83.5
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTAT	AGCCCAAGCGAGACTTACTA	47.6	50.0	58.0	58.2	801-1141 bp	1.0	82.5