Hotspot

psbK-psbI

LSC · 8170-8755 · 586 bp

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Pi 0.121

MarkerSeek score 85.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 586
variable_sites: 119
pi: 0.121
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGAAAAGTTTGAGAGTAAGCA	50.0	36.4	57.9	56.2	573-639 bp	1.0	61.5
psbK-psbI_p2	AGCATTTGTTTGGCAAGCTG	ACGAAAAGTTTGAGAGTAAGCA	45.0	36.4	58.1	56.2	580-646 bp	1.0	61.0
psbK-psbI_p3	ATTTGTTTGGCAAGCTGCTG	ACGAAAAGTTTGAGAGTAAGCA	45.0	36.4	58.1	56.2	577-643 bp	1.0	61.0
psbK-psbI_p4	CATTTGTTTGGCAAGCTGCT	ACGAAAAGTTTGAGAGTAAGCA	45.0	36.4	58.1	56.2	578-644 bp	1.0	61.0
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	ACGAAAAGTTTGAGAGTAAGCA	45.0	36.4	58.3	56.2	570-636 bp	1.0	59.5