Hotspot

rpl32-trnL-UAG

SSC · 114052-114906 · 855 bp

Back to Withania

Pi 0.0266

MarkerSeek score 71.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 855
variable_sites: 69
pi: 0.0266
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CAGTGAATCGAACGGGGATT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1570-1686 bp	0.625	68.9
rpl32-trnL-UAG_p2	CAGTGAATCGAACGGGGATT	GTCCCGAAGAAATCCAACGA	50.0	50.0	58.0	57.9	1906-2029 bp	0.625	68.4
rpl32-trnL-UAG_p3	CAGTGAATCGAACGGGGATT	CCCGAAGAAATCCAACGAGT	50.0	50.0	58.0	57.9	1904-2027 bp	0.625	68.4
rpl32-trnL-UAG_p4	CAGTGAATCGAACGGGGATT	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.0	58.1	2057-2180 bp	0.625	68.1
rpl32-trnL-UAG_p5	CAGTGAATCGAACGGGGATT	TGAAGTAGCAAAGCCTTGGG	50.0	50.0	58.0	58.1	2058-2181 bp	0.625	68.1