Hotspot

rpl36-rps8

LSC · 81243-81691 · 449 bp

Back to Withania

Pi 0.0314

MarkerSeek score 61.70

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 449
variable_sites: 37
pi: 0.0314
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	CAAGAAACTACTCGACCCCG	GCCCGGGAATTGAGAGAATT	55.0	50.0	58.0	57.9	1346-1467 bp	1.0	87.0
rpl36-rps8_p2	ACTTCCACTGTAGTGTCCGA	GCCCGGGAATTGAGAGAATT	50.0	50.0	58.0	57.9	1719-1840 bp	1.0	86.6
rpl36-rps8_p3	TTCCACTGTAGTGTCCGAGT	GCCCGGGAATTGAGAGAATT	50.0	50.0	58.0	57.9	1717-1838 bp	1.0	86.6
rpl36-rps8_p4	AATGCTGCATCTCTTCCGAG	GCCCGGGAATTGAGAGAATT	50.0	50.0	58.0	57.9	1505-1626 bp	1.0	86.5
rpl36-rps8_p5	CTACTCGACCCCGTACATCT	GCCCGGGAATTGAGAGAATT	55.0	50.0	58.0	57.9	1339-1460 bp	0.875	81.6