Hotspot

rps4-trnT-UGU

LSC · 47588-47951 · 364 bp

Back to Withania

Pi 0.0228

MarkerSeek score 61.90

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 364
variable_sites: 23
pi: 0.0228
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GTTAGTCCTGGTAAAGCCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.6	57.9	484-492 bp	1.0	81.2
rps4-trnT-UGU_p2	GTTAGTCCTGGTAAAGCCCC	AGATTTCTGTGATGTGGGCC	55.0	50.0	57.6	57.9	511-519 bp	1.0	80.9
rps4-trnT-UGU_p3	GTTAGTCCTGGTAAAGCCCC	ATGGTCATCGGTTCGATTCC	55.0	50.0	57.6	57.8	452-460 bp	1.0	80.7
rps4-trnT-UGU_p4	TTAGTCCTGGTAAAGCCCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.7	57.9	483-491 bp	1.0	78.8
rps4-trnT-UGU_p5	TTAGTCCTGGTAAAGCCCCC	AGATTTCTGTGATGTGGGCC	55.0	50.0	58.7	57.9	510-518 bp	1.0	78.5