Hotspot

trnS-trnG

LSC · 8675-9303 · 629 bp

Back to Withania

Pi 0.0161

MarkerSeek score 65.40

Primer available yes

Label trnS-trnG

Feature Metadata

label_name: trnS-trnG
feature_type: igs
region: LSC
length_bp: 629
variable_sites: 30
pi: 0.0161
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnG_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	680-724 bp	1.0	58.8
trnS-trnG_p2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	45.0	39.1	58.0	57.3	683-727 bp	1.0	57.8
trnS-trnG_p3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	682-726 bp	1.0	57.8
trnS-trnG_p4	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	729-773 bp	1.0	57.7
trnS-trnG_p5	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	45.0	36.4	58.0	56.2	681-725 bp	1.0	56.9