Hotspot

trnK-rps16

LSC · 4362-5037 · 676 bp

Back to Withania

Pi 0.0254

MarkerSeek score 65.40

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 676
variable_sites: 49
pi: 0.0254
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	CCCTTTCAGGATCAGTCGTG	AAAAGGGTATGTTGCTGCCA	55.0	45.0	58.0	58.0	1487-1509 bp	0.875	78.9
trnK-rps16_p2	CCTTTCAGGATCAGTCGTGG	AAAAGGGTATGTTGCTGCCA	55.0	45.0	58.0	58.0	1486-1508 bp	0.875	78.9
trnK-rps16_p3	TCCCTTTCAGGATCAGTCGT	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.1	58.0	1488-1510 bp	0.875	78.6
trnK-rps16_p4	TCAGGATCAGTCGTGGTCTT	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.1	58.0	1482-1504 bp	0.875	78.5
trnK-rps16_p5	TTCAGGATCAGTCGTGGTCT	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.1	58.0	1483-1505 bp	0.875	78.5