Hotspot

trnH-psbA

LSC · 82-532 · 451 bp

Back to Withania

Pi 0.0578

MarkerSeek score 83.60

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 451
variable_sites: 83
pi: 0.0578
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	475-521 bp	1.0	90.4
trnH-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	489-535 bp	1.0	90.3
trnH-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	488-534 bp	1.0	90.3
trnH-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	490-536 bp	1.0	90.3
trnH-psbA_p5	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	55.0	55.0	58.1	58.3	524-570 bp	1.0	88.3