Hotspot

rps3

Genome · 86744-87397 · 654 bp

Back to Wisteriopsis

Pi 0.00153

MarkerSeek score 59.60

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: Genome
length_bp: 654
variable_sites: 1
pi: 0.00153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TCGAAAGATGGAACGTTAAAGA	AGCAGCAAATCCTAGTCACA	36.4	45.0	55.8	56.8	810 bp	1.0	40.1
rps3_p2	TCGAAAGATGGAACGTTAAAGA	AGCAGCAAATCCTAGTCACAA	36.4	42.9	55.8	57.6	810 bp	1.0	40.1
rps3_p3	TCGAAAGATGGAACGTTAAAGAT	AGCAGCAAATCCTAGTCACA	34.8	45.0	56.3	56.8	810 bp	1.0	40.1
rps3_p4	CGAAAGATGGAACGTTAAAGA	AGCAGCAAATCCTAGTCACA	38.1	45.0	54.3	56.8	809 bp	1.0	40.1
rps3_p5	TCGAAAGATGGAACGTTAAAG	AGCAGCAAATCCTAGTCACA	38.1	45.0	54.3	56.8	810 bp	1.0	40.1