Hotspot

ndhF

Genome · 130080-132314 · 2235 bp

Back to Wisteriopsis

Pi 0.000895

MarkerSeek score 49.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2235
variable_sites: 2
pi: 0.000895
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACCGAACCCTTTACAAAAGAGA	GCGGATAACCCCAATTTAGGT	40.9	47.6	57.6	58.1	2366 bp	1.0	52.2
ndhF_p2	ACCGAACCCTTTACAAAAGAGA	AGCGGATAACCCCAATTTAGG	40.9	47.6	57.6	57.8	2367 bp	1.0	50.9
ndhF_p3	ACCGAACCCTTTACAAAAGAGA	TGAAGCTTTTCGGATGAGACA	40.9	42.9	57.6	57.6	2415 bp	1.0	49.4
ndhF_p4	ACCGAACCCTTTACAAAAGAG	GCGGATAACCCCAATTTAGGT	42.9	47.6	56.1	58.1	2366 bp	1.0	48.3
ndhF_p5	ACCGAACCCTTTACAAAAGAGAT	GCGGATAACCCCAATTTAGGT	39.1	47.6	58.0	58.1	2366 bp	1.0	47.4