Hotspot

rpl32-ndhF

Genome · 129311-130079 · 769 bp

Back to Wisteriopsis

Pi 0.0013

MarkerSeek score 59.50

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: Genome
length_bp: 769
variable_sites: 1
pi: 0.0013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AAGGCTTTCAATGCTGTCCA	TGTTGCCGTGGGAAAAAGTA	45.0	45.0	58.0	57.9	963 bp	1.0	79.6
rpl32-ndhF_p2	AGGCTTTCAATGCTGTCCAA	TGTTGCCGTGGGAAAAAGTA	45.0	45.0	58.0	57.9	962 bp	1.0	79.6
rpl32-ndhF_p3	AAGGCTTTCAATGCTGTCCA	CGGGGTGAAAGGTATGATCC	45.0	55.0	58.0	57.7	909 bp	1.0	78.0
rpl32-ndhF_p4	AGGCTTTCAATGCTGTCCAA	CGGGGTGAAAGGTATGATCC	45.0	55.0	58.0	57.7	908 bp	1.0	78.0
rpl32-ndhF_p5	AGGCTTTCAATGCTGTCCAA	TTTGTTGCCGTGGGAAAAAG	45.0	45.0	58.0	57.7	964 bp	1.0	77.7