Hotspot

ycf1-rps15

Genome · 118333-118823 · 491 bp

Back to Wisteriopsis

Pi 0
MarkerSeek score 60.40
Primer available yes
Label ycf1-rps15

Feature Metadata

label_name
ycf1-rps15
feature_type
igs
region
Genome
length_bp
491
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-rps15_p1 ACCCATAATGGAAGCCGTTT TCAACTGATCCGGTGTTTTCT 45.0 42.9 57.5 57.5 605-625 bp 1.0 66.5
ycf1-rps15_p2 ACCCATAATGGAAGCCGTTT ACTGATCCGGTGTTTTCTTCT 45.0 42.9 57.5 57.2 602-622 bp 1.0 64.2
ycf1-rps15_p3 ACCCATAATGGAAGCCGTTT TCAACTGATCCGGTGTTTTC 45.0 45.0 57.5 56.0 605-625 bp 1.0 62.3
ycf1-rps15_p4 ACCCATAATGGAAGCCGTTT CAACTGATCCGGTGTTTTCT 45.0 45.0 57.5 56.0 604-624 bp 1.0 62.2
ycf1-rps15_p5 ACCCATAATGGAAGCCGTTT ACTGATCCGGTGTTTTCTTCTT 45.0 40.9 57.5 57.9 602-622 bp 1.0 61.7