Hotspot

psbE-petL

Genome · 67282-68803 · 1522 bp

Back to Wisteriopsis

Pi 0.00131
MarkerSeek score 59.50
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
Genome
length_bp
1522
variable_sites
2
pi
0.00131
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 TGACAAATAACCAACCCGCA AACCGCTAGTAGAAAACCGA 45.0 45.0 58.0 56.6 1679-1680 bp 1.0 68.7
psbE-petL_p2 TGACAAATAACCAACCCGCA ACCGCTAGTAGAAAACCGAA 45.0 45.0 58.0 56.6 1678-1679 bp 1.0 68.7
psbE-petL_p3 CAACCCGCAATGAATAGGGA AACCGCTAGTAGAAAACCGA 50.0 45.0 57.9 56.6 1668-1669 bp 1.0 68.5
psbE-petL_p4 CAACCCGCAATGAATAGGGA ACCGCTAGTAGAAAACCGAA 50.0 45.0 57.9 56.6 1667-1668 bp 1.0 68.5
psbE-petL_p5 AGAACGTTCTCCTGTGCTTC ACCGCTAGTAGAAAACCGAA 50.0 45.0 57.8 56.6 1590-1591 bp 1.0 67.6