Hotspot

trnG-GCC-psbZ

Genome · 26129-26942 · 814 bp

Back to Wisteriopsis

Pi 0.00246
MarkerSeek score 57.80
Primer available yes
Label trnG-GCC-psbZ

Feature Metadata

label_name
trnG-GCC-psbZ
feature_type
igs
region
Genome
length_bp
814
variable_sites
2
pi
0.00246
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-psbZ_p1 CGTCTTCTCCTTGGCAAAGA CTTCTCCTGATGGTTGGTCG 50.0 55.0 57.8 58.0 968 bp 1.0 78.7
trnG-GCC-psbZ_p2 TACATAAAAAGGCAGGGGCG CTTCTCCTGATGGTTGGTCG 50.0 55.0 58.2 58.0 1008 bp 1.0 78.1
trnG-GCC-psbZ_p3 CGTCTTCTCCTTGGCAAAGA TTGCTTCTCCTGATGGTTGG 50.0 50.0 57.8 57.8 971 bp 1.0 77.2
trnG-GCC-psbZ_p4 CGTCTTCTCCTTGGCAAAGA TCCTGATGGTTGGTCGAGTA 50.0 50.0 57.8 57.8 964 bp 1.0 77.0
trnG-GCC-psbZ_p5 CGTCTTCTCCTTGGCAAAGA GCTTCTCCTGATGGTTGGTC 50.0 55.0 57.8 58.3 969 bp 1.0 76.8