Hotspot

rbcL-atpB

Genome · 7191-7972 · 782 bp

Back to Wisteriopsis

Pi 0.00128

MarkerSeek score 59.60

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: Genome
length_bp: 782
variable_sites: 1
pi: 0.00128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	AACACCAGCTTTGAACCCAA	GGCGATACGTCCCAGATTTT	45.0	50.0	58.1	58.1	904-905 bp	1.0	78.6
rbcL-atpB_p2	AACACCAGCTTTGAACCCAA	CCTGGGGGAAAAGCTACATC	45.0	55.0	58.1	57.9	945-946 bp	1.0	78.6
rbcL-atpB_p3	AACACCAGCTTTGAACCCAA	GGACCGATTATTTGGGCGAT	45.0	50.0	58.1	58.1	918-919 bp	1.0	78.2
rbcL-atpB_p4	GCTTTGAACCCAACACTTGC	GGCGATACGTCCCAGATTTT	50.0	50.0	58.4	58.1	897-898 bp	1.0	76.3
rbcL-atpB_p5	GCTTTGAACCCAACACTTGC	CCTGGGGGAAAAGCTACATC	50.0	55.0	58.4	57.9	938-939 bp	1.0	76.3