Hotspot

ndhA

Genome · 119145-121448 · 2304 bp

Back to Wisteria

Pi 0.0087

MarkerSeek score 56.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: Genome
length_bp: 2304
variable_sites: 20
pi: 0.0087
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CACCGGGTTTTATCAATTTGCA	ACCCAGTTACCATAAGGAACA	40.9	42.9	58.1	56.3	2521-2525 bp	1.0	42.2
ndhA_p2	GTCCACCGGGTTTTATCAAT	ACCCAGTTACCATAAGGAACA	45.0	42.9	55.7	56.3	2524-2528 bp	1.0	40.8
ndhA_p3	ACCGGGTTTTATCAATTTGCA	ACCCAGTTACCATAAGGAACA	38.1	42.9	56.7	56.3	2520-2524 bp	1.0	40.8
ndhA_p4	CACCGGGTTTTATCAATTTGCA	AACCCAGTTACCATAAGGAACA	40.9	40.9	58.1	57.0	2522-2526 bp	1.0	40.8
ndhA_p5	CACCGGGTTTTATCAATTTGCA	TGAACCCAGTTACCATAAGGA	40.9	42.9	58.1	56.0	2524-2528 bp	1.0	40.8