Hotspot

trnL-UAG-rpl32

Genome · 127174-127843 · 670 bp

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Pi 0.0231
MarkerSeek score 62.80
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
Genome
length_bp
670
variable_sites
14
pi
0.0231
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AAGTAGCAAAGCCTTGGGTC TGGACAGCATTGAAAGCCTT 50.0 45.0 58.1 58.0 1302 bp 0.5 59.3
trnL-UAG-rpl32_p2 AAGTAGCAAAGCCTTGGGTC TTGGACAGCATTGAAAGCCT 50.0 45.0 58.1 58.0 1303 bp 0.5 59.3
trnL-UAG-rpl32_p3 GAAGTAGCAAAGCCTTGGGT TGGACAGCATTGAAAGCCTT 50.0 45.0 58.1 58.0 1303 bp 0.5 59.3
trnL-UAG-rpl32_p4 GAAGTAGCAAAGCCTTGGGT TTGGACAGCATTGAAAGCCT 50.0 45.0 58.1 58.0 1304 bp 0.5 59.3
trnL-UAG-rpl32_p5 ATGGGATTGTAGAGCGGGTA TGGACAGCATTGAAAGCCTT 50.0 45.0 57.9 58.0 1361 bp 0.5 59.2