Hotspot

psaC-ndhD

Genome · 124123-124242 · 120 bp

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Pi 0.0417

MarkerSeek score 76.50

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: Genome
length_bp: 120
variable_sites: 5
pi: 0.0417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	CAGAGGACTGTGTTGGTTGT	ATTAAGGAACCCCCGGCTAT	50.0	50.0	57.7	58.2	298 bp	1.0	77.3
psaC-ndhD_p2	CAGAGGACTGTGTTGGTTGT	CCCGGCTATTGGCAAAACTA	50.0	50.0	57.7	58.2	287 bp	1.0	76.8
psaC-ndhD_p3	CAGAGGACTGTGTTGGTTGT	AATTAAGGAACCCCCGGCTA	50.0	50.0	57.7	58.4	299 bp	1.0	75.5
psaC-ndhD_p4	CAGAGGACTGTGTTGGTTGT	TTAAGGAACCCCCGGCTATT	50.0	50.0	57.7	58.4	297 bp	1.0	75.5
psaC-ndhD_p5	CAGAGGACTGTGTTGGTTGT	AAAATTAAGGAACCCCCGGC	50.0	50.0	57.7	58.4	301 bp	1.0	75.2